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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential position in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two additional splice variants have been reported but specific nucleotide sequence details will not be yet readily available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further more probable optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind for the epitope about the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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